Filing Details

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 13D Under the Securities Exchange Act of 1934 (Amendment No. 1) Nanosphere, Inc (Name of Issuer) Common (Title of Class of Securities) 63009F105 (CUSIP Number) Richard C. Perkins, 730 Lake St E, Wayzata, MN 55391, 952-473-8367 (Name, Address and Telephone Number of Person Authorized) 05/17/2016 (Date of Event which Requires Filing of this Statement) If the filing person has previously filed a statement on Schedule 13G to report the acquisition that is the subject of this Schedule 13D, and is filing this schedule because of ss240.13d-1(e), 240.13d-1(f) or 240.13d-1(g), check the following box. / / Note: Schedules filed in paper format shall include a signed original and five copies of the schedule, including all exhibits. See s240.13d-7 for other parties to whom copies are to be sent. The remainder of this cover page shall be filled out for a reporting person's initial filing on this form with respect to the subject class of securities, and for any subsequent amendment containing information which would alter disclosures provided in a prior cover page. The information required in the remainder of this cover page shall not be deemed to be "filed" for the purpose of Section 18 of the Securities Exchange Act of 1934 ("Act") or otherwise subject to the liabilities of that section of the Act but shall be subject to all other provisions of the Act (however, see the Notes). Potential persons who are to respond to the collection of information contained in this form are not required to respond unless the form displays a currently valid OMB control number.

CUSIP No. 63009F105 1.Names of Reporting Persons. I.R.S. Identification Nos. of above persons (entities only). Perkins Capital Management, Inc. 41-1501962 2.Check the Appropriate Box if a Member of a Group (See Instructions) (a) / / (b) / / 3.SEC Use Only 4.Source of Funds (See Instructions) OO 6.Citizenship or Place of Organization 730 East Lake Street, Wayzata, Minnesota 55391 Number of 7.Sole Voting Power 4,500 Shares Beneficially 8.Shared Voting Power owned 0 by Each 9.Sole Dispositive Power 1,540,244 Reporting Person 10.Shared Dispositive Power With 0 11. Aggregate Amount Beneficially Owned by Each Reporting Person 1,540,244 12. Check if the Aggregate Amount in Row (11) Excludes Certain Shares 13. Percent of Class Represented by Amount in Row (11) 10.2% 14. Type of Reporting Person (See Instructions) IAGENERAL INSTRUCTIONS Item 1. Security and Issuer: Nanosphere, Inc 4088 Commercial Avenue Northbrook, IL 60062 Item 2. Identity and Background: (a)Name: Perkins Capital Management, Inc. (b)Business Address: 730 Lake St E, Wayzata, MN 55391 c)Present Principal occupation or employment and the name, principal business and address of any corporation or other organization in which such employment is conducted; Perkins Capital Management, Inc. is a federally registered investment adviser. (d) Whether or not, during the last five years, such person has been convicted in a criminal proceeding (excluding traffic violation or similar misdemeanors) and, if so, give the dates, nature of conviction, name and location of court, and penalty imposed, or other disposition of the case; No named person has been convicted in a criminal proceeding (e) Whether or not, during the last five years, such person was a party to a civil proceeding of a judicial or administrative body of competent jurisdiction and as a result of such proceeding was or is subject to a judgment, decree or final order enjoining future violations of, or prohibiting or mandating activities subject to, federal or state securities laws or finding any violation with respect to such laws; and, if so, identify and describe such proceedings and summarize the terms of such judgement, decree or final order; and No named person has been party to such incidents (f)Citizenship Perkins Capital Managment, Inc. is a Minnesota Corporation Item 3. Source and Amount of Funds or Other Consideration The funds used in purchasing shares on behalf of clients of Perkins Capital Management, Inc. come from various client accounts in which Perkins Capital Management, Inc. disclaims beneficial ownership./s/Richard C. Perkins Signature Richard C. Perkins/Executive Vice President Name/Title